PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning
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چکیده
منابع مشابه
PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.
A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide convenient priming sites for polymerase chain reaction amplification. In this way, as much as 1 microgram of D...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.22.9027